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Purification, concentration and evaluation of rabies virus antigen
Comparative Immunology, Microbiology and Infectious Diseases
|January 1, 1982
Summary
Large-scale production of rabies virus antigen relies on ultrafiltration and density gradient centrifugation. A modified antibody binding test effectively evaluated inactivated antigen concentrates during production.
Area of Science:
- Virology
- Biotechnology
- Immunology
Background:
- Numerous methods for concentrating and purifying rabies virus antigen exist.
- Ultrafiltration and density gradient centrifugation are the most viable for large-scale production.
Purpose of the Study:
- To evaluate the suitability of ultrafiltration and density gradient centrifugation for large-scale rabies virus antigen production.
- To adapt and utilize an antibody binding test for in-process control of antigen concentrate quality.
Main Methods:
- Large-scale concentration and purification using ultrafiltration and density gradient centrifugation.
- Modification of the Arko, Wiktor, and Sikes antibody binding test.
- Application of the modified test as an in-process control for inactivated antigen concentrates.
Main Results:
- Ultrafiltration and density gradient centrifugation proved effective for large-scale rabies virus antigen production.
- The modified antibody binding test served as a reliable in-process control for evaluating antigen concentrates.
- Practical experiences demonstrated the utility of these methods.
Conclusions:
- Ultrafiltration and density gradient centrifugation are suitable for the industrial-scale production of rabies virus antigen.
- The adapted antibody binding test is a valuable tool for quality control during the manufacturing process.
- These optimized procedures facilitate efficient and reliable rabies antigen production.