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Activator proteins for lipoprotein lipase from bovine plasma: preparation by adsorption to Intralipid
A method has been developed for preparation from bovine plasma of the apolipoproteins which activate lipoprotein lipase. The method is based on the known ability of these proteins to transfer from the plasma lipoproteins to the lipid droplets of Intralipid. The protein lipid complexes were harvested and washed by low speed centrifugation, the proteins were then delipidated and separated by gel filtration in 6 M guanidine hydrochloride followed by anion exchange chromatography in 6 M urea. By this method batches of 5-1. bovine plasma can readily be processed, yielding about 20 mg of purified activators. These separated into three fractions, the properties of which suggest that they are isoproteins.
A method has been developed for preparation from bovine plasma of the apolipoproteins which activate lipoprotein lipase. The method is based on the known ability of these proteins to transfer from the plasma lipoproteins to the lipid droplets of Intralipid. The protein lipid complexes were harvested and washed by low speed centrifugation, the proteins were then delipidated and separated by gel filtration in 6 M guanidine hydrochloride followed by anion exchange chromatography in 6 M urea. By this method batches of 5-1. bovine plasma can readily be processed, yielding about 20 mg of purified activators. These separated into three fractions, the properties of which suggest that they are isoproteins.