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Related Experiment Videos

Growth factor from tumor cells

N Harada, M Okamoto, Y Ogata

    Gan
    |June 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified optimal conditions for assaying a tumor growth factor using 3T3 cells, which proved most sensitive. Partial purification revealed a thermolabile, high-molecular-weight growth factor from AH-130 tumor cells.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Oncology

    Background:

    • Tumor cells release growth factors that influence proliferation.
    • Identifying and characterizing these factors is crucial for understanding cancer progression.

    Purpose of the Study:

    • To investigate suitable conditions for assaying tumor growth factors.
    • To partially purify and characterize a growth factor from AH-130 tumor cells.

    Main Methods:

    • Assay development using baby hamster kidney cells, human embryonic lung cells, and Swiss Albino mouse 3T3 cells.
    • Partial purification via ammonium sulfate fractionation, DEAE-cellulose chromatography, and Sephacryl S-300 gel filtration.
    • Assessment of growth factor activity and properties.

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    Main Results:

    • The 3T3 cell line demonstrated higher sensitivity to growth-stimulating activity compared to other cell lines.
    • AH-130 tumor cells yielded a more potent growth factor fraction than Yoshida sarcoma cells.
    • The partially purified growth factor was thermolabile and estimated to have a molecular weight of approximately 80,000 Da.

    Conclusions:

    • 3T3 cells are suitable for sensitive assaying of tumor growth factors.
    • A high-molecular-weight, thermolabile growth factor was identified in AH-130 tumor cells.