Characteristics of human chondrocyte cultures in completely defined medium

Chondrocytes derived from normal human adult articular cartilage were established and maintained for over 5 months in a completely defined medium without the addition of serum or any other growth factors. At the end of 5 months, these cells were still metabolically active. The cells incorporated [3H] thymidine into DNA, incorporated [35S] sulfate into proteoglycans, and exhibited lysosomal enzyme activities. The 35S-labeled proteoglycans isolated from the culture medium had elution profiles on high pressure liquid chromatography (HPLC) similar to those observed for proteoglycans from other mammalian sources. This self-contained growth competence may reflect a need produced by the unusual avascular and alymphatic character of articular cartilage.