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Updated: Oct 21, 2026

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity
Published on: September 16, 2011
Subthreshold and suboptimal desensitization of human basophils. II. Nonspecificity and irreversibility of
Abstract:
Desensitization in human basophils was induced by suboptimal levels of antihuman IgE or antigen under normal calcium buffer conditions. Low doses of anti-IgE triggered only 0-12% histamine release during a 3-hour preincubation, yet initiated up to 100% desensitization upon rechallenge. After 24-27 h preincubation with allergen a, the cells did not regain releasability when rechallenged with either allergen a, allergen b or anti-IgE. Low dose, IgE-mediated desensitization in human basophils may lead to an irreversible and nonspecific inhibition of degranulation.
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