Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variable major proteins of Borrellia hermsii

A G Barbour, S L Tessier, H G Stoenner

    The Journal of Experimental Medicine
    |November 1, 1982
    PubMed
    Summary

    Borrelia hermsii exhibits antigenic variation through changes in its variable outer surface protein (pI). This study identifies pI as the serotype-specific antigen responsible for Borrelia hermsii

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Borrelia miyamotoi infection in nature and in humans.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2015
    Same author

    Ticks have R2 retrotransposons but not the consensus transposon target site of other arthropods.

    Insect molecular biology·2005
    Same author

    OspA immunization decreases transmission of Borrelia burgdorferi spirochetes from infected Peromyscus leucopus mice to larval Ixodes scapularis ticks.

    Vector borne and zoonotic diseases (Larchmont, N.Y.)·2003
    Same author

    Antigen polymorphism in Borrelia hermsii, a clonal pathogenic bacterium.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Identification and characterization of a homologue of the pro-inflammatory cytokine Macrophage Migration Inhibitory Factor in the tick, Amblyomma americanum.

    Insect molecular biology·2001
    Same author

    Non-heritable change of a spirochaete's phenotype by decoration of the cell surface with exogenous lipoproteins.

    Molecular microbiology·2001

    Area of Science:

    • Microbiology
    • Immunology
    • Molecular Biology

    Background:

    • Borrelia hermsii causes relapsing fever and exhibits antigenic variation.
    • Understanding the molecular basis of this variation is crucial for disease control.

    Purpose of the Study:

    • To identify the specific antigens responsible for antigenic variation in Borrelia hermsii.
    • To characterize the proteins involved in serotype specificity.

    Main Methods:

    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to fractionate bacterial proteins.
    • Western blotting with serotype-specific antisera and monoclonal antibodies.
    • Immunofluorescence assays and radioiodination to determine protein localization.

    Main Results:

    • Two major proteins, pI and pII, were identified in Borrelia hermsii lysates.
    • Protein pI showed molecular weight variation between serotypes, while pII remained constant.
    • Serotype-specific antisera and monoclonal antibodies recognized only homologous pI proteins.
    • pI proteins were localized to the bacterial surface.

    Conclusions:

    • The variable pI proteins are the serotype-specific antigens of Borrelia hermsii.
    • Changes in pI proteins likely underlie the antigenic variation observed during relapsing fever.

    Related Experiment Videos