Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variable opacity of glycogen in routine electron micrographs

L E Thornell, M Sjöström, U Karlsson

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |September 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Successful complete oral rehabilitation of a patient with osteopetrosis with extensive pre-treatments, bone grafts, dental implants and fixed bridges: a multidisciplinary case report.

    BMC oral health·2023
    Same author

    Real-life data revealed strong immunity against SARS-CoV-2 variants BA.4/BA.5 in long-term care facility residents previously infected with BA.1/BA.2.

    The Journal of hospital infection·2023
    Same author

    Influenza A subtype H3N2 is associated with an increased risk of hospital dissemination - an observational study over six influenza seasons.

    The Journal of hospital infection·2023
    Same author

    Fragmentomic analysis of circulating tumor DNA-targeted cancer panels.

    Annals of oncology : official journal of the European Society for Medical Oncology·2023
    Same author

    A mobile app for the treatment of female mixed and urgency incontinence: a cost-effectiveness analysis in Sweden.

    International urogynecology journal·2022
    Same author

    Dynamic allosteric communication pathway directing differential activation of the glucocorticoid receptor.

    Science advances·2020

    Glycogen presence in frog muscle nerve terminals was confirmed using electron microscopy. Proper cytochemical staining is crucial for accurately assessing glycogen organization within cells.

    Area of Science:

    • Cell Biology
    • Neuroscience
    • Biochemistry

    Background:

    • Glycogen serves as an energy reserve in various cell types.
    • Understanding glycogen's role in nerve terminals is important for neuronal function.
    • Previous studies have varied in their depiction of glycogen in nerve cells.

    Purpose of the Study:

    • To identify and characterize glycogen within the nerve terminals of frog muscle.
    • To evaluate the impact of different preparation techniques on glycogen visualization.
    • To emphasize the importance of cytochemical methods in glycogen analysis.

    Main Methods:

    • Transmission electron microscopy (TEM) was employed.
    • Periodic acid-thiosemicarbazide-silverproteinate (PA-TCH-SP) staining was used for glycogen detection.

    Related Experiment Videos

  • Uranyl acetate and lead citrate (UAc-PbCi) staining was also performed on serial sections.

    • Main Results:

    • Glycogen was successfully identified in the nerve terminals of the reticular zone of frog muscle.
    • The appearance of glycogen in UAc-PbCi-stained terminals varied, suggesting a dependence on the preparative procedure.
    • PA-TCH-SP staining provided a reliable method for glycogen visualization.

    Conclusions:

    • Cytochemical identification is essential for the accurate assessment of glycogen organization.
    • Preparative methods significantly influence the observed morphology of glycogen in nerve terminals.
    • This study highlights the need for standardized cytochemical techniques in ultrastructural glycogen analysis.