Design and synthesis of new reactive fluorescent dyes for cytofluorometry

Our purpose has been to synthesize reactive fluorescent compounds with different excitation and/or emission spectra that can be used for multiple fluorescence analysis in combination with the "natural" cell fluorescence exhibited by reduced coenzyme (NAD(P)H) or flavins. Synthesis of condensation products of p-bis(2-chloroethyl)-amino-benzaldehyde is described. Their absorption spectra range from 360 to 480 nm and their emission spectra lie between 520 and 590 mm. Examples of cytological applications are given.