Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytochemical method to localize acidic nuclear proteins

A C Dhar, C K Shah

    Stain Technology
    |May 1, 1982
    PubMed
    Summary

    Fast green FCF dye specifically binds to acidic nuclear proteins in plant cells at pH 5.0. This method distinguishes acidic proteins from basic proteins in Limnophyton obtusifolium nuclear structures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    In vitro propagation of guayule (Parthenium argentatum) - a rubber yielding shrub.

    Plant cell reports·2013
    Same author

    Education and imaging: Hepatobiliary and pancreatic: liver abscess caused by an ingested foreign body.

    Journal of gastroenterology and hepatology·2009
    Same author

    Persistent complete heart block following acute rheumatic fever in a 12 year old girl.

    The Journal of the Association of Physicians of India·1993
    Same author

    Role of ascorbic acid in auxin induced cell elongation--a cytochemical study.

    Histochemistry·1980
    Same author

    Use of photoresistor for the measurement of light absorption in cytophotometer.

    Indian journal of experimental biology·1975
    Same author

    Histological studies of cortisone and A.C.T.H. treated rabbits after fracture.

    Journal of experimental medical sciences·1965

    Area of Science:

    • Plant cell biology
    • Molecular biology
    • Biochemistry

    Background:

    • Identifying specific proteins within cellular structures is crucial for understanding biological functions.
    • Histochemical staining techniques are vital for visualizing cellular components.

    Purpose of the Study:

    • To determine the specificity of Fast Green FCF (FCF) staining for acidic nuclear proteins.
    • To differentiate between acidic and basic nuclear proteins in Limnophyton obtusifolium.

    Main Methods:

    • Sections of young flower buds of Limnophyton obtusifolium were stained with Fast Green FCF across a pH range (2.6-9.0).
    • Control preparations involved acetylation, acid hydrolysis, alkali treatment, methylation, and deamination to verify staining specificity.
    • Staining intensity and localization (nuclear vs. cytoplasmic) were analyzed at different pH levels.

    Main Results:

    • Fast Green FCF staining was localized to the nucleus at pH 5.0 and pH 8.0, with no cytoplasmic reaction.
    • Staining intensity was greater at pH 5.0 than at pH 8.0.
    • Control treatments confirmed that Fast Green FCF at pH 5.0 specifically binds to acidic nuclear proteins, while binding at pH 8.0 indicates basic proteins.

    Conclusions:

    • Fast Green FCF at pH 5.0 is a specific stain for acidic nuclear proteins.
    • The differential staining at pH 5.0 and pH 8.0 allows for the distinction between acidic and basic nuclear proteins in plant tissues.

    Related Experiment Videos