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Updated: Sep 11, 2026

Electron Cryotomography of Bacterial Cells
Published on: May 6, 2010
Preparation of biological cryosections for analytical electron microscopy
Abstract:
A cooling chain for studies of ultrastructure and elemental composition of cryofixed biological cells and tissues is described. The technique is demonstrated for yeast cells. The preparation steps such as cryofixation, cryosectioning, transfer into the electron microscope as well as imaging and X-ray microanalysis in the STEM are discussed with respect to the present possibilities and problems. Whereas freeze-dried cryosections can be studied routinely, imaging of the ultrastructure and measurement of the elemental distribution in frozen-hydrated sections turn out to be limited.
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Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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