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Related Experiment Videos

Trapped plasma in the microhematocrit

T C Pearson, D L Guthrie

    American Journal of Clinical Pathology
    |November 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Trapped plasma in red blood cells was studied in normal individuals and patients with various conditions. Findings are relevant for accurate packed cell volume (PCV) determination and red blood cell index calculations.

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    Area of Science:

    • Hematology
    • Clinical Pathology

    Background:

    • Trapped plasma in microhematocrit red blood cell columns can affect packed cell volume (PCV) measurements.
    • Understanding trapped plasma is crucial for accurate red blood cell indices.

    Purpose of the Study:

    • To investigate the amount of trapped plasma in red blood cell columns.
    • To analyze trapped plasma levels in normal individuals and patients with specific hematological conditions.

    Main Methods:

    • Microhematocrit centrifugation technique was used.
    • Analysis of trapped plasma in red blood cell columns from 25 normal individuals and 102 patients.

    Main Results:

    • Mean trapped plasma was 1.53% in normal individuals.
    • Patient groups (sickle cell disease, iron deficiency, hereditary spherocytosis) showed mean values from 1.41% to 1.82%.

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  • An inverse correlation was observed between trapped plasma and mean corpuscular hemoglobin (MCH) in iron-deficiency patients (MCH ≤ 25.0 pg).

    • Conclusions:

    • Trapped plasma levels vary slightly between normal individuals and patients with specific anemias.
    • The inverse correlation in iron deficiency suggests a relationship between MCH and trapped plasma.
    • These findings impact the precise determination of PCV and related red blood cell indices.