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A cyclohexanecarboxylic acid utilizing yeast: isolation, identification, and nutritional characteristics

K Higuchi, H Obata, T Tokuyama

    Canadian Journal of Microbiology
    |August 1, 1982
    PubMed
    Summary

    A novel yeast strain, Trichosporon cutaneum KUY-6A, was identified for its ability to metabolize cyclohexanecarboxylic acid as its primary energy source. This yeast demonstrates broad substrate utilization, including various cyclic and aliphatic carboxylic acids.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Microbial degradation of organic compounds is crucial for nutrient cycling.
    • Cyclic carboxylic acids represent a unique class of substrates for microbial metabolism.
    • Isolation of novel microorganisms with specific metabolic capabilities is essential for biotechnological applications.

    Purpose of the Study:

    • To isolate and identify a yeast strain capable of utilizing cyclohexanecarboxylic acid.
    • To characterize the metabolic versatility of the isolated yeast strain.
    • To determine the growth yield on cyclohexanecarboxylic acid.

    Main Methods:

    • Enrichment culture techniques using cyclohexanecarboxylic acid as the sole carbon source.
    • Taxonomical identification of the isolated yeast strain.

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  • Growth experiments on various carboxylic acids and related compounds.
  • Determination of cell dry weight yield.

    • Main Results:

    • Isolation of yeast strain KUY-6A, identified as Trichosporon cutaneum.
    • Strain KUY-6A efficiently utilized cyclohexanecarboxylic acid as a sole carbon and energy source.
    • The yeast demonstrated growth on a wide range of cyclic (cyclopentanecarboxylic, cycloheptanecarboxylic acids, cyclopentanone, cyclohexanone, cyclopentanol) and aliphatic (butyric, valeric, caproic) monocarboxylic acids, as well as dicarboxylic (succinic, glutaric, adipic, pimelic, suberic) and aromatic (benzoic, hydroxybenzoic) acids.
    • A cell dry weight yield of 0.75 g per gram of cyclohexanecarboxylic acid was achieved.
    • Thiamine showed slight growth stimulation, but no other vitamins were required.

    Conclusions:

    • Trichosporon cutaneum KUY-6A is a robust yeast capable of metabolizing cyclohexanecarboxylic acid.
    • This strain possesses a broad substrate range, indicating significant metabolic flexibility.
    • The high yield suggests potential for biotechnological applications in the degradation or conversion of cyclic carboxylic acids.