Seasonal fluctuations in river bacteria as measured by multivariate statistical analysis of continuous cultures

Forty-eight continuous culture enrichments were performed on summer and winter water samples from two contrasting rivers. The cell output from each chemostat was dependent on the temperature and nitrogen concentration of each enrichment. The diversity of the populations from the continuous cultures, as assessed by species diversity analysis, was always greater than populations obtained on agar plates. However, the species isolated exclusively by continuous culture in these experiments were not unique to the chemostat. All of these species had been isolated at some time on plates directly. High nitrogen concentrations were found to decrease diversity. Populations sampled during the winter were influenced primarily by the concentration of nitrogen. Summer isolates were affected mainly by the temperature of the culture medium. The nutritional versatility of the population was not affected by enrichment of the continuous culture.