Related Experiment Videos
Corynebacterium kutscheri invasiveness of the gastrointestinal tract in young mice
Abstract:
Corynebacterium kutscheri invasiveness of the tissues of the gastrointestinal tract in mice was investigated by the fluorescent antibody technique. The invasion was clearly recognized in 3-week-old mice infected intragastrically along with gastric mucin and a subcutaneous dose of cortisone; 10(7) organisms caused a notable invasion of the gastric and duodenal tissues. Furthermore, marked bacterial invasion of the large intestine appeared in mice challenged perorally with 7 x 10(7) organisms.
Insights
Corynebacterium kutscheri invasion of mouse gastrointestinal tissues was studied. Intragastric infection with mucin and cortisone in 3-week-old mice showed significant gastric and duodenal tissue invasion.
Area of Science:
- Microbiology
- Immunology
- Gastroenterology
Background:
- Corynebacterium kutscheri is a Gram-positive bacterium.
- Understanding bacterial invasiveness is crucial for disease pathogenesis.
Purpose of the Study:
- To investigate the invasiveness of Corynebacterium kutscheri in the gastrointestinal tract of mice.
- To identify factors influencing bacterial tissue colonization.
Main Methods:
- Fluorescent antibody technique was used to visualize bacterial invasion.
- Mice were infected intragastrically with Corynebacterium kutscheri.
- Cortisone administration and gastric mucin were employed to enhance invasion.
Main Results:
- Significant invasion of gastric and duodenal tissues was observed in 3-week-old mice infected intragastrically with 10(7) organisms.
- Marked bacterial invasion of the large intestine occurred in mice challenged perorally with 7 x 10(7) organisms.
Conclusions:
- Corynebacterium kutscheri can invade the gastrointestinal tract tissues in mice.
- Age, intragastric administration with mucin, and cortisone influence bacterial invasiveness.