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Tumor-promoting phorbol esters induce macrophage differentiation from bone marrow precursors
Experimental Cell Biology
|January 1, 1982
Summary
The tumor-promoting phorbol diester, 12-0-tetradecanoyl-phorbol-13-acetate (TPA), stimulates bone marrow cells to proliferate and differentiate into macrophages. This process mimics the effects of macrophage colony-stimulating activity, suggesting TPA
Area of Science:
- Cell Biology
- Immunology
- Hematopoiesis
Background:
- Colony-stimulating activity regulates hematopoietic cell growth and differentiation.
- Phorbol esters are known tumor promoters that can affect cell behavior.
Purpose of the Study:
- To investigate the effects of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) on bone marrow cells.
- To compare TPA-induced changes with those induced by macrophage colony-stimulating activity.
Main Methods:
- Culturing rat and mouse bone marrow cells in liquid culture.
- Treating cultures with TPA.
- Assessing DNA synthesis, cell proliferation, adherence, lysozyme secretion, Fc receptor expression, and cytotoxicity.
Main Results:
- TPA stimulated DNA synthesis and cell proliferation in bone marrow cells.
- TPA enhanced cell adherence, lysozyme secretion, and Fc receptor expression.
- TPA-induced cells exhibited lymphokine-induced cytotoxicity.
Conclusions:
- TPA mimics the effects of macrophage colony-stimulating activity.
- TPA selectively stimulates mononuclear phagocyte precursors to proliferate and differentiate into macrophages.