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One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography

C Bruck, D Portetelle, C Glineur

Journal of Immunological Methods

|September 30, 1982

Abstract:

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

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