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Postnatal developmental changes in fucosyltransferase activity in rat brain

M Léon, P Broquet, R Morélis

    Journal of Neurochemistry
    |December 1, 1982
    PubMed
    Summary

    GDP-fucose:asialofetuin fucosyltransferase activity in rat brain shows significant postnatal development, peaking around 21 days. This key enzyme

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    Area of Science:

    • Neuroscience
    • Biochemistry
    • Developmental Biology

    Background:

    • Postnatal development of the rat brain involves complex enzymatic changes.
    • GDP-fucose:asialofetuin fucosyltransferase plays a role in glycosylation processes crucial for brain maturation.

    Purpose of the Study:

    • To investigate the developmental profile of GDP-fucose:asialofetuin fucosyltransferase activity in the postnatal rat brain.
    • To examine the influence of age on the stimulation of this enzymatic activity by Triton X-100.
    • To compare the developmental patterns of GDP-fucose pyrophosphatase and fucosidase with fucosyltransferase.

    Main Methods:

    • Measurement of GDP-fucose:asialofetuin fucosyltransferase activity in rat brain homogenates at various postnatal ages.
    • Assay of GDP-fucose pyrophosphatase and fucosidase activity during the same developmental period.
    • Assessment of Triton X-100 stimulation effects on fucosyltransferase activity across different ages.

    Main Results:

    • Enzymatic activity was low in early postnatal life, increasing significantly to a peak around 21 days.
    • The increase exhibited two rapid phases: between 3-7 days and 17-21 days, with a slower rise from 7-17 days.
    • Triton X-100 stimulation of fucosyltransferase activity increased with age, while pyrophosphatase and fucosidase levels remained stable.

    Conclusions:

    • Rat brain GDP-fucose:asialofetuin fucosyltransferase undergoes substantial postnatal development, suggesting a critical role in brain maturation.
    • The specific developmental pattern indicates distinct regulatory mechanisms during early and later postnatal periods.
    • Age-dependent modulation of enzyme activity by Triton X-100 highlights developmental changes in enzyme-surfactant interactions.

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