'Free' postsynaptic-like densities in normal adult brain: their occurrence, distribution, structure and association...

Samples of cerebral cortex (parietal and occipital) and thalamic nuclei (ventrobasal, posterolateral, dorsal lateral geniculate) from normal, adult, aldehyde perfusion fixed mice and rats were examined by electron microscopy for the presence of free postsynaptic-like densities (FPSDs). FPSDs are plaques of intracellular paramembranous electron-dense material, ultrastructurally indistinguishable from postsynaptic densities, but not aligned with a presynaptic specialization. In a systematic survey of the neuropil around 6000 neuronal perikarya, 250 FPSDs were encountered. Almost all of these were within dendritic spines and shafts and about 90% of them were apposed by a neuronal perikaryon, the remainder by a dendritic shaft. In every case a subsurface cistern (SSC) was present in the cell body or dendrite apposed to the FPSD, and was flattened along the extent of the FPSD. In none of the material were the FPSDs associated, even remotely, with degenerating elements, suggesting that they are not formed by degeneration of presynaptic boutons. The incidence of FPSD-SSC complexes was higher in thalamus than in cerebral cortex which, together with previous observations indicating their absence from normal cerebellar cortex, suggests significant regional variations in distribution. It is suggested that FPSDs might represent synaptic precursors perhaps induced to form as a response to loss (possibly age-dependent loss) of synaptic contacts on a neuron and that the SSCs are somehow involved in maintaining the FPSDs and/or preparing them for innervation by adjacent axon terminals to form new synaptic contacts.