Chromosomal localization of the Moloney sarcoma virus mouse cellular (c-mos) sequence

Related Experiment Video

Updated: Aug 8, 2026

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
10:10

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Published on: May 28, 2017

D Swan, M Oskarsson, D Keithley

    Journal of Virology
    |November 1, 1982
    PubMed

    Insights

    Researchers identified the location of the mos gene in mice. Using DNA hybridization, they pinpointed the mos gene to mouse chromosome 4, advancing cancer gene research.

    Area of Science:

    • Genetics
    • Molecular Biology
    • Oncology

    Background:

    • The mos oncogene, derived from Moloney sarcoma virus (v-mos), plays a role in cellular transformation.
    • Understanding the chromosomal location of oncogenes is crucial for genetic mapping and cancer research.

    Purpose of the Study:

    • To determine the specific chromosomal location of the mouse cellular mos gene.
    • To utilize hybrid cell lines for gene mapping.

    Main Methods:

    • Employing the v-mos oncogene as a genetic probe.
    • Hybridizing the probe to restricted DNA from mouse-Chinese hamster hybrid cell lines.
    • Analyzing the presence or absence of the mos gene in correlation with known karyotypes.

    Main Results:

    • The mos gene was detected in specific mouse-Chinese hamster hybrid cell lines.
    • Correlation between the presence of the mos gene and the karyotype of hybrid cell lines was established.
    • The cellular mos gene was definitively assigned to mouse chromosome 4.

    Conclusions:

    • The cellular mos gene is located on mouse chromosome 4.
    • This finding contributes to the genetic map of the mouse genome.
    • Precise gene localization aids in understanding oncogene function and potential therapeutic targets.

    More Related Videos

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction08:01

    Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction

    Published on: November 20, 2018

    An Integrated Approach for Microprotein Identification and Sequence Analysis09:37

    An Integrated Approach for Microprotein Identification and Sequence Analysis

    Published on: July 12, 2022

    Related Concept Videos

    Mouse Models of Cancer Study02:43

    Mouse Models of Cancer Study

    Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
    The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...