A simple method for repetitive blood sampling from rats

A convenient, reliable method for obtaining serial blood samples from rats is described. A simple cannula device is maintained in the ventral caudal artery of lightly etherized rats. Sequential blood samples of desired volume can be taken as frequently as needed without wastage. The technique is minimally traumatic to the animal and does not require extensive preparatory time. Since airtight blood samples can be taken, the technique can be used for pharmacokinetic studies of volatile compounds.