Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Melatonin synthesis by rabbit platelets

J M Launay, B J Lamaître, H P Husson

    Life Sciences
    |October 4, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of operator performance during oocyte retrievals: residents' learning curve and continuous monitoring of senior physicians.

    BMC medical education·2021
    Same author

    Late onset diplopia reversed by an epidural blood patch.

    International journal of obstetric anesthesia·2020
    Same author

    Surgical treatment of septate uterus in cases of primary infertility and before assisted reproductive technologies.

    Journal of gynecology obstetrics and human reproduction·2018
    Same author

    [In vitro fertilization versus conversion to intrauterine insemination in patients with poor response to controlled ovarian hyperstimulation].

    Gynecologie, obstetrique, fertilite & senologie·2018
    Same author

    Inflammation versus mechanical stretch biomarkers over time in acutely decompensated heart failure with reduced ejection fraction.

    International journal of cardiology·2016
    Same author

    Raphe-mediated signals control the hippocampal response to SRI antidepressants via miR-16.

    Translational psychiatry·2012
    Same journal

    Corrigendum to "Adipose stem cells-derived microvesicles and chicken egg-derived exosomes attenuate cardiac ischemia/reperfusion injury through AKT/ERK/Nrf2/HO-1 axis to inhibit apoptosis and inflammation and restore autophagy" [Life Sci. 395 (2026) 124364].

    Life sciences·2026
    Same journal

    MAGED1 stabilizes NEUROD1 to promote Per3 expression in the pineal gland.

    Life sciences·2026
    Same journal

    TNF-centered network pharmacology and molecular modeling of selected Andrographis paniculata compounds in hypertension.

    Life sciences·2026
    Same journal

    Retraction notice to "Beneficial effect of Calculus Bovis Sativus on 17α-ethynylestradiol-induced cholestasis in the rat" [Life Sci. 113 (2014) 22-30].

    Life sciences·2026
    Same journal

    Soluble PD-1 drives renal fibrosis in CKD by disrupting immune homeostasis: Therapeutic mitigation via a targeted sPD-1 sequestration strategy.

    Life sciences·2026
    Same journal

    METTL1 promotes hepatic steatosis by mediating m<sup>7</sup>G modification of ALOX15B mRNA.

    Life sciences·2026
    See all related articles

    Rabbit platelets can convert serotonin (5-HT) into melatonin. This study identifies melatonin as the key product, demonstrating platelet capacity for this important biosynthesis.

    Area of Science:

    • Biochemistry
    • Pharmacology
    • Cell Biology

    Background:

    • Platelets are known for their role in hemostasis and thrombosis.
    • Serotonin (5-hydroxytryptamine, 5-HT) is a neurotransmitter and a potent vasoconstrictor stored in platelets.
    • The metabolic fate of serotonin within platelets is not fully elucidated.

    Purpose of the Study:

    • To investigate the metabolic conversion of serotonin (5-HT) in rabbit platelets.
    • To identify potential metabolites of 5-HT produced by platelets.
    • To determine if platelets possess the enzymatic machinery for melatonin biosynthesis.

    Main Methods:

    • Incubation of reserpinized rabbit platelets with tritiated 5-HT (3H-5HT).
    • Extraction of labeled compounds using alkaline-chloroform.

    Related Experiment Videos

  • Analysis by thin-layer chromatography (TLC) and mass spectrometry (MS).
  • Confirmation using radioimmunoassay (RIA).

    • Main Results:

    • Platelets converted 3H-5HT into chloroform-extractable labeled compounds.
    • The primary product co-migrated with authentic melatonin on TLC.
    • Mass spectrometry and radioimmunoassay confirmed the identity of the labeled product as melatonin.
    • Enzymes serotonin N-acetyltransferase and hydroxyindole-O-methyltransferase were implicated.

    Conclusions:

    • Rabbit platelets possess the enzymatic capacity to synthesize melatonin from serotonin (5-HT).
    • This study demonstrates an in vitro pathway for melatonin production within platelets.
    • The findings suggest a novel role for platelets in melatonin biosynthesis.