Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tunicamycin-resistant mutations in mouse FM3A cells

H Koyama, D Ayusawa, M Okawa

    Mutation Research
    |October 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Two isoforms of acid phosphatase secreted by tobacco protoplasts: differential effect of brefeldin A on their secretion.

    Plant cell reports·2013
    Same author

    Effects of the autonomic nervous system on functional neuroimaging: analyses based on the vector autoregressive model.

    Advances in experimental medicine and biology·2012
    Same author

    Demethylation of MAGE promoters during gastric cancer progression.

    British journal of cancer·2004
    Same author

    Promoter methylation status of tumor suppressor and tumor-related genes in neoplastic and non-neoplastic gastric epithelia.

    Histology and histopathology·2004
    Same author

    Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae.

    Molecular genetics and genomics : MGG·2003
    Same author

    Hypermethylation of the hMLH1 gene promoter in solitary and multiple gastric cancers with microsatellite instability.

    British journal of cancer·2002

    Tunicamycin antibiotic resistance was studied in mouse cells. Researchers developed a selection system to identify resistant mutations, finding they occur randomly and are stably inherited, offering insights into glycoprotein synthesis.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Genetics

    Background:

    • Tunicamycin is an antibiotic inhibiting glycoprotein oligosaccharide synthesis.
    • It suppresses the growth of cultured mouse mammary carcinoma FM3A cells at concentrations above 0.1 microgram/ml.

    Purpose of the Study:

    • To develop a single-step selection system for detecting tunicamycin-resistant mutations in FM3A cells.
    • To characterize the frequency, inheritance, and resistance levels of these mutations.

    Main Methods:

    • Developed a selection system using tunicamycin at optimal concentrations (1-1.2 micrograms/ml).
    • Utilized fluctuation analysis (Luria-Delbrück) to determine mutation rates.
    • Isolated and characterized spontaneous and MNNG-induced mutant lines.
    • Assessed tunicamycin resistance using D10 values and [3H]mannose incorporation.

    Related Experiment Videos

    Main Results:

    • Tunicamycin-resistant mutants appeared at frequencies of 10(-4) to 10(-5) in unmutagenized cells, increasing over 50-fold after MNNG mutagenesis.
    • Mutation rate was determined to be 1.2 x 10(-6) per cell per generation.
    • Isolated mutant lines inherited resistance for over 60 generations.
    • Mutants exhibited 12- to 26-fold higher resistance (D10 value) and over 30-fold higher resistance in [3H]mannose incorporation compared to wild-type cells.
    • Tunicamycin resistance showed co-dominant inheritance in cell hybrids.

    Conclusions:

    • A robust selection system for tunicamycin resistance in FM3A cells was established.
    • Tunicamycin-resistant mutations arise randomly and are stably inherited.
    • The findings provide a basis for studying mechanisms of tunicamycin resistance and its impact on glycoprotein synthesis.