GLC determination of methoxsalen in human serum and urine

A simple, sensitive and specific GLC method for the quantitation of methoxsalen in human serum and urine is described. The procedure involves chloroformic extraction of the active ingredient and the internal standard, trioxsalen, followed by GLC determination, using flame ionization detector and 3% OV on a Gas chromosorb W column. Sensitivity was 0.1 microgram/ml and 0.2 microgram/ml for serum and urine, respectively. Methoxsalen contents of serum of rats treated with this drug are reported.