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The neurotoxicity of organotin: behavioural changes in rats
Abstract:
The neurotoxic action of the fungicide Tinestan 60 WP (containing 60% triphenyltin acetate) was examined in male rats. Due to daily doses as small doses as 6 and 0.6 mg/kg daily administered over six weeks the exploratory activity in maze was diminished, the animals made significantly more errors. There was no difference between the poisoned and the control groups in the speed of acquisition of the conditioned avoidance responses, however the extinction was significantly slower, as a sign of slower development of active inhibition. Tested in stress situation the behaviour of poisoned animals altered, as a sign of decreased habituation. It is concluded that Tinestan crosses the blood-brain barrier, and induces diminished plasticity of the CNS.
Insights
Triphenyltin acetate, a fungicide found in Tinestan 60 WP, impairs rat behavior and learning. Even low doses reduce exploratory activity and habituation, indicating neurotoxicity.
Area of Science:
- Neuroscience
- Toxicology
- Environmental Health
Background:
- Fungicides are widely used in agriculture.
- Triphenyltin compounds are known for their toxicity.
- The neurotoxic effects of Tinestan 60 WP require further investigation.
Purpose of the Study:
- To investigate the neurotoxic effects of Tinestan 60 WP in male rats.
- To assess the impact of triphenyltin acetate on behavior and learning.
- To determine if Tinestan 60 WP crosses the blood-brain barrier.
Main Methods:
- Male rats were administered daily doses of Tinestan 60 WP (6 mg/kg and 0.6 mg/kg) for six weeks.
- Behavioral tests included maze exploration, conditioned avoidance responses, and habituation to stress.
- Neurotoxicity was assessed by observing changes in activity, learning, inhibition, and habituation.
Main Results:
- Low doses of Tinestan 60 WP significantly reduced exploratory activity and increased errors in maze tasks.
- While learning speed was unaffected, poisoned rats showed slower extinction of conditioned responses, indicating impaired active inhibition.
- Poisoned animals exhibited altered behavior under stress, suggesting decreased habituation.
- These findings suggest Tinestan 60 WP crosses the blood-brain barrier.
Conclusions:
- Tinestan 60 WP exhibits significant neurotoxic effects in male rats.
- The fungicide impairs central nervous system (CNS) plasticity, affecting learning and behavioral adaptation.
- Triphenyltin acetate exposure can lead to diminished cognitive functions and altered stress responses.