Effects of shipping on the immune function in mice

Area of Science:

• Immunology
• Animal Physiology
• Stress Response

Background:

• Animal transportation is common in research.
• Shipping can induce physiological stress.
• Understanding stress effects on immune function is vital for animal welfare and experimental validity.

Purpose of the Study:

• To investigate the impact of shipping stress on immunologic functions in mice.
• To assess physiological stress markers, such as corticosterone levels.
• To determine the necessary acclimatization period for newly arrived animals.

Main Methods:

• Mice were shipped via truck and plane, with environmental monitoring (temperature, light).
• Immunologic function was assessed using foot pad tests, hemagglutination assays, and plaque-forming cell assays.
• Corticosterone concentrations were measured using a competitive protein-binding technique.

Main Results:

• Shipment, regardless of mode, caused significant increases in corticosterone levels upon arrival.
• Immune function assays showed a marked decrease at arrival.
• Both corticosterone levels and immune function returned to baseline levels within 48 hours post-shipment.

Conclusions:

• Shipping stress elevates corticosterone and suppresses immune function in mice.
• A minimum 48-hour stabilization period is essential for newly arrived mice before experimental use.
• This highlights the importance of acclimatization for reliable research outcomes.