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Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
07:28

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

Published on: July 10, 2013

The effect of accelerated release of rat neutrophil polymorphonuclear leucocytes from bone marrow on cell

W H Lu, D M Williams

    British Journal of Experimental Pathology
    |August 1, 1982
    PubMed

    Insights

    Accelerated release of polymorphonuclear leucocytes (PMN) during inflammation does not impair their migration. This study found no difference in PMN cell emigration into inflammatory sites, regardless of bone marrow transit time.

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • Polymorphonuclear leucocytes (PMN) are crucial immune cells involved in inflammatory responses.
    • Their function relies on effective migration to sites of injury or infection.
    • Premature release from bone marrow may alter PMN function.

    Purpose of the Study:

    • To investigate the impact of shortened bone marrow transit time on PMN migratory abilities.
    • To determine if accelerated PMN release affects their emigration during inflammation.

    Main Methods:

    • Healthy rats received (methyl-3H) thymidine to label bone marrow cells.
    • An inflammatory stimulus (Brain Heart Infusion/Proteose Peptone) was administered.
    • Isotope incorporation in blood and peritoneal exudates was measured over 12 hours using liquid scintillation counting.

    Main Results:

    • No significant difference in isotope incorporation was observed between blood and peritoneal exudates within the first 12 hours.
    • This indicates that PMN emigration into inflammatory lesions was not affected.

    Conclusions:

    • Accelerated release of PMN from bone marrow does not impair their ability to migrate into inflammatory sites within the initial 12-hour period.
    • The shortened bone marrow transit time, in this context, does not compromise PMN extravasation during the early inflammatory response.

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