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Related Experiment Videos

Hexagonal surface array in a protein-secreting bacterium, Bacillus brevis 47

N Tsukagoshi, H Yamada, A Tsuboi

    Biochimica Et Biophysica Acta
    |December 8, 1982
    PubMed
    Summary
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    Bacillus brevis 47 cell walls feature a hexagonal protein array. A mutant lacking a key protein altered this array structure, impacting its unit cell composition.

    Area of Science:

    • Microbiology
    • Structural Biology
    • Biochemistry

    Background:

    • Bacillus brevis 47 is a protein-secreting bacterium.
    • Its cell wall possesses a distinct hexagonal array of structural units.

    Purpose of the Study:

    • To investigate the structural organization of the Bacillus brevis 47 cell surface array.
    • To understand the role of major cell wall proteins in maintaining this array structure.

    Main Methods:

    • Analysis of cell wall proteins using molecular weight determination.
    • Electron microscopy and image processing of wild-type and mutant cell envelopes.
    • Comparison of surface array structures between wild-type and mutant strains.

    Main Results:

    Related Experiment Videos

  • Wild-type Bacillus brevis 47 cell walls contain 150,000 and 130,000 molecular weight proteins, forming a hexagonal array with six units per cell.
  • A mutant strain (47-57) lacking the 130,000 protein showed altered array structure with three units per cell, despite maintaining the same lattice constant.
  • The array structure and composition were consistent across different growth conditions.

    • Conclusions:

    • The 130,000 molecular weight protein is crucial for the complete formation of the Bacillus brevis 47 surface array structure.
    • The study proposes two models for the surface array based on the observed differences between wild-type and mutant strains.