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[The influence of sodium nitrite upon the physical functions of mice]
Abstract:
Male mice of ICR-strain (four weeks old) were divided into seven groups, of each five animals. Sodium nitrite (NaNO2), dissolved in the physiological saline solution, was administered orally at doses of 250, 220, 110, 80, 50 and 5 mg per kg, everyday during successive seven days to each group. The forced running distance was monitored by means of the treadmill (Eiken-model) during the period of the administration and recovery. All the mice which received 250 mg/kg dose died after the single shot. The capability of muscular exercise was found decreased particularly in the groups which received 110 and 80 mg/kg of NaNO2. Mean methemoglobin level (Met-Hb) in the blood of male mice was about 1.0%. Mean Met-Hb levels were increased to about 1.2% and 3.8% after the oral administration of NaNO2 at the dose of 110 mg/kg everyday for successive 7 and 21 days, respectively. Met-Hb levels were measured by means of modified Evelyn-Malloy's method. In the groups which received 110 mg/kg or less, no significant changes in the body weight as well as the other physical functions were observed, as compared with those received only physiological saline solutions.
Insights
Sodium nitrite (NaNO2) significantly impairs exercise capacity in male mice, with higher doses causing mortality. This study highlights NaNO2
Area of Science:
- Toxicology
- Physiology
- Pharmacology
Context:
- Sodium nitrite (NaNO2) is an industrial chemical and food additive.
- Its physiological effects, particularly on exercise performance and blood parameters, require thorough investigation.
- Understanding these effects is crucial for assessing potential health risks.
Purpose:
- To investigate the impact of oral sodium nitrite administration on the physical exercise capacity and methemoglobin levels in male ICR mice.
- To determine dose-dependent toxicological effects of NaNO2.
Summary:
- Male ICR mice were administered varying doses of NaNO2 orally for seven days.
- High doses (250 mg/kg) were lethal, while moderate doses (110 and 80 mg/kg) significantly reduced exercise performance.
- NaNO2 administration increased mean methemoglobin (Met-Hb) levels, particularly at 110 mg/kg over 7 and 21 days.
Impact:
- This research demonstrates the detrimental effects of sodium nitrite on exercise capability and induces methemoglobinemia.
- Findings suggest potential risks associated with NaNO2 exposure, impacting physical performance and oxygen transport.
- Provides critical data for toxicological assessments and safety guidelines regarding NaNO2.