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Ascorbic acid uptake in guinea pig intestinal mucosa

L T Patterson, D L Nahrwold, R C Rose

    Life Sciences
    |December 13, 1982
    PubMed
    Summary

    Guinea pigs absorb ascorbic acid (vitamin C) in their intestines via a sodium-dependent carrier mechanism. This process involves active transport across the intestinal brush border, indicating efficient vitamin C uptake.

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    Area of Science:

    • Cell Biology
    • Gastroenterology
    • Nutritional Science

    Background:

    • Ascorbic acid (vitamin C) is essential for various physiological functions.
    • Understanding its absorption mechanisms is crucial for nutritional science and medicine.

    Purpose of the Study:

    • To investigate the in vitro cellular accumulation of ascorbic acid in guinea pig distal intestinal mucosa.
    • To elucidate the transport mechanism of ascorbic acid across the intestinal epithelium.

    Main Methods:

    • In vitro study using 14C-ascorbic acid in guinea pig distal intestinal mucosa.
    • Assessing tissue/media concentration ratios and diffusion rates.
    • Evaluating the effect of sodium, metabolic depletion, and unlabeled ascorbic acid on uptake.
    • Investigating counter-transport with unlabeled ascorbic acid and mannitol.

    Main Results:

    • Achieved tissue/media concentration ratios of at least 5 for ascorbic acid.
    • Ascorbic acid diffused out of poisoned tissue with a half-time of ~10 minutes.
    • Uptake was 97% lower without sodium and reduced by 67% with unlabeled ascorbic acid.
    • Demonstrated counter-transport, indicating a carrier-mediated process.

    Conclusions:

    • Ascorbic acid uptake in guinea pig intestine is a carrier-mediated process.
    • Sodium is essential for active ascorbic acid transport.
    • Evidence supports a brush border carrier mechanism for ascorbic acid absorption.

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