[Effect of the carbon sources and metabolic inhibitors on endopolymethylgalacturonase secretion in Aspergillus...

The mycelium of Aspergillus foetidus was grown for 48 h, washed off, and used to study the effect of various carbon sources on the synthesis and secretion of endopolymethylgalacturonase (endo-PMG). Sucrose, glucose, glucose-6-phosphate, fructose, pectin and mannitol were shown to favour the enzyme secretion from the mycelium. The enzyme accumulation in the medium was inhibited when such inhibitors of metabolism as sodium azide and fluoride, 2,4-dinitrophenol and N,N'-dicyclohexyl carbodiimide were added, together with glucose, to the phosphate buffer. The secretion of endo-PMG from the mycelium of Aspergillus foetidus is presumed to be an energy-dependent process.