[Dynamics of microtubule reconstitution around the cell center in cultured cells after cooling]

After cooling, microtubules are absent in the cell center of PE interphase cells. Following the transfer of the cells to the thermostate (37 degrees), a reconstitution of the microtubule network begins in the cell center. The number of microtubules, diverging radially from centrioles, reaches the maximum after 12-15 minutes and then falls down to the normal level. Within the first 12 minutes in the most part of cells electron-dense microtubule organazing centers are seen around centrioles which disappear later. It is supposed that microtubules are associated with the center only during their growth. Thus, the number of microtubules associated with it at any moment is not the number of microtubules in the whole cell, but it shows the intensity of their renewal.