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[Arthrobacter globiformis - a new yeast-lysing bacterium]
Zeitschrift Fur Allgemeine Mikrobiologie
|January 1, 1982
Summary
A novel bacterium, Arthrobacter globiformis, produces a yeast-lytic enzyme complex. This enzyme complex, containing beta-glucanase and alpha-mannanase, effectively lyses specific yeast species by degrading their cell walls.
Area of Science:
- Microbiology
- Enzymology
Context:
- Compost environments can harbor microorganisms with unique enzymatic capabilities.
- Baker's yeast (Saccharomyces cerevisiae) is a common component in compost enrichment.
Purpose:
- To isolate and identify a bacterium capable of lysing yeast cells.
- To characterize the yeast lytic enzyme complex produced by the isolated bacterium.
- To determine the optimal conditions for enzyme production and identify the target yeast species.
Summary:
- Arthrobacter globiformis was isolated from compost and found to produce a yeast lytic enzyme complex.
- The enzyme complex, containing beta-glucanase and alpha-mannanase, was purified and characterized.
- Optimal enzyme synthesis was achieved using specific carbon sources like beta-glucan and alpha-mannan, or whole baker's yeast cells.
- The enzyme complex effectively lysed several yeast species, including Pichia membranaefaciens and Candida guilliermondii, by releasing spheroplasts.
Impact:
- This research identifies a potential source of novel yeast-lytic enzymes for biotechnological applications.
- Understanding the specificities of these enzymes can aid in targeted yeast cell lysis.
- The study contributes to the knowledge of microbial enzymes involved in cell wall degradation.