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Human mononuclear phagocytes differentiation antigens

A Dimitriu-Bona, R J Winchester, G R Burmester

    Advances in Experimental Medicine and Biology
    |January 1, 1982
    PubMed
    Summary
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    Mononuclear phagocyte lineage cells express multiple cell surface antigens that change during maturation and in vitro culture. These antigenic patterns help define cell states in normal and leukemic populations, including acute myeloid leukemia (AML).

    Area of Science:

    • Immunology
    • Cell Biology
    • Hematology

    Background:

    • The mononuclear phagocyte lineage comprises cells with diverse functions and phenotypes.
    • Understanding cell surface antigen expression is crucial for defining cell lineages and states.

    Purpose of the Study:

    • To analyze cell surface antigenic phenotypes of the mononuclear phagocyte lineage using monoclonal antibodies.
    • To investigate antigen modifications during cell maturation and in vitro culture.
    • To correlate antigenic patterns with cell maturity in normal and leukemic states, particularly acute myeloid leukemia (AML).

    Main Methods:

    • Utilized 10 distinct monoclonal antibodies for cell surface antigenic analysis.
    • Examined antigen expression on various normal and leukemic cell populations.

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  • Studied antigen modulation in blood monocytes during in vitro culture and activation.

    • Main Results:

    • Mononuclear phagocyte lineage cells co-express multiple distinct cell surface antigens.
    • Cell maturation involves qualitative and/or quantitative changes in surface antigens.
    • In vitro culture and activation modulate antigen expression, notably MoP-15.
    • Defined antigenic expression patterns correspond to maturational states.
    • AML cells exhibit antigen expression patterns related to their maturity, which change during culture.

    Conclusions:

    • Cell surface antigen analysis provides a framework for understanding mononuclear phagocyte lineage differentiation.
    • Antigenic profiling can distinguish different maturational stages and activation states.
    • These findings offer insights into the immunophenotypic characterization of normal and malignant hematopoietic cells, including AML.