Clonal proliferation of peritoneal exudate cells from New Zealand black mice: age-related changes

Proliferation and differentiation of peritoneal exudate colony-forming cells (PE-CFC) were examined in BALB/c, C57BL/6, and NZB mice; NZB mice provided an experimental animal model for studies of autoimmunity. At 1 and 12 months of age, the number of PE-CFC from NZB mice was significantly less than the number of PE-CFC for other tested strains, whereas the number of clusters formed was reduced only in the older NZB mice. In contrast, 1- to 12-month-old BALB/c and C57BL/6 mice showed no significant age or strain-dependent alterations in PE-CFC. Although the latency period preceding colony growth remained constant for all strains and ages, significant variation in colony size was observed for NZB mice; the largest colonies were noted in the oldest group. We also observed age-associated variations in colony morphology; therefore, age- and strain-dependent differences may exist in the differentiation or functional attributes of PE-CFC subpopulations. The results specifically indicate that NZB mice have age-related alterations in PEC proliferative and differentiation capacities.