Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quality control limits for the agar overlay disk diffusion antimicrobial susceptibility test

A L Barry, R E Badal

    Journal of Clinical Microbiology
    |December 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Something New-Nitric Acid a Remedy for Stings and Poisons.

    The Southern medical record·2022
    Same author

    Antimicrobial resistance and resistance mechanisms of Enterobacteriaceae in ICU and non-ICU wards in Europe and North America: SMART 2011-2013.

    Journal of global antimicrobial resistance·2016
    Same author

    Trending eight years of in vitro activity of ertapenem and comparators against Escherichia coli from intra-abdominal infections in North America--SMART 2002-2009.

    Journal of chemotherapy (Florence, Italy)·2011
    Same author

    Susceptibility of European Escherichia coli clinical isolates from intra-abdominal infections, extended-spectrum β-lactamase occurrence, resistance distribution, and molecular characterization of ertapenem-resistant isolates (SMART 2008-2009).

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2011
    Same author

    Cefuroxime, an in vitro Comparison with Six Other Cephalosporins.

    Proceedings of the Royal Society of Medicine·2010
    Same author

    Ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests.

    The Journal of antimicrobial chemotherapy·2009

    Quality control tests show that the agar overlay method is comparable to the Kirby-Bauer technique for antimicrobial susceptibility testing. Established quality control limits for Kirby-Bauer can be applied to the agar overlay method.

    Area of Science:

    • Clinical microbiology
    • Antimicrobial susceptibility testing

    Background:

    • The Kirby-Bauer method is a standard for antimicrobial susceptibility testing.
    • The agar overlay technique is an alternative method for determining bacterial susceptibility to antibiotics.

    Purpose of the Study:

    • To compare the results of the agar overlay technique with the Kirby-Bauer method.
    • To determine if existing quality control limits are applicable to the agar overlay technique.

    Main Methods:

    • Replicate control tests were conducted using both agar overlay and Kirby-Bauer techniques.
    • Two sources of Mueller-Hinton agar were utilized for the tests.

    Main Results:

    • Mean zone diameters showed minor differences (1–3 mm) between the two methods.

    Related Experiment Videos

  • The National Committee for Clinical Laboratory Standards (NCCLS) quality control limits for the Kirby-Bauer method were found to be applicable to the agar overlay technique.

    • Conclusions:

    • The agar overlay technique provides comparable results to the Kirby-Bauer method for antimicrobial susceptibility testing.
    • NCCLS quality control limits established for the Kirby-Bauer method can be effectively used for the agar overlay technique, simplifying quality control procedures.