Related Experiment Video
Updated: Jul 29, 2026

Inducible and Reversible Dominant-negative (DN) Protein Inhibition
Published on: January 7, 2019
Digitoxin metabolism by rat, mouse, and rabbit: NADPH requirement and spironolactone effect
Abstract:
Digitoxin metabolism in rat, mouse, and rabbit liver homogenates was compared. The rabbit metabolized the compound most extensively, yielding digitoxigenin as major hydrolysis product. Rat and mouse both produced digitoxigenin bisdigitoxoside as major product. Metabolite formation was enhanced by NADPH in all three species. Spironolactone induced the hydrolysis of digitoxin in the rat, but had no effect in the mouse or rabbit. The mechanism of the protective effect of spironolactone in the mouse does not involve induction of digitoxin hydrolysis; in the rabbit, rapid inactivation of spironolactone accounts for the lack of induction.
More Related Videos
11:04Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
Published on: September 3, 2020
02:44Use of Micropipette-Guided Drug Administration as an Alternative Method to Oral Gavage in Rodent Models
Published on: July 26, 2024
Related Concept Videos
Therapeutic Drug Monitoring: Affecting Factors
Therapeutic Drug Monitoring: Drug Analysis Methods