Successful in vivo experiments in sheep using a new nonhydrolyzable enzyme reactor resistant against tryptic...

Blockage of E-amino groups of asp-ase bound to cellulose by succinylation results in a greatly enhanced resistance against tryptic digestion by "plasma enzymes." Plasmapheresis in sheep by plasma filters connected to asp-ase Cellulose cartridges led to decrease of asp-NH2 and glut-NH2 in plasma over 21 hrs with undetectable enzyme leakage from the solid phase.