A freeze-fracture study of the human nail plate

This study of freeze-fracture replicas of human nail plate fragments demonstrates that keratin filaments have a width of about 100 A. The 'marginal band' is probably formed by a row of particles, 50 A in diameter, arranged along the inner surface of the plasma membrane. These particles are often masked by keratin filaments. In nonjunctional areas, the plasma membranes are devoid of membrane-associated particles. The only type of junction observed is the desmosome. Even though the modes of keratinization are different, the structures of the keratin pattern, of cell membranes, and of the desmosomes are identical in replicas of wholly keratinized nail and human stratum corneum cells. The ultrastructural differences observed appear to be related to the barrier function carried out by the epidermis and by its desquamation process.