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Microtubule function and its relation to cellular development and the yeast cell cycle in Wangiella dermatitidis

C W Jacobs, P J Szaniszlo

    Archives of Microbiology
    |December 1, 1982
    PubMed

    Insights

    Nocodazole, a microtubule inhibitor, blocked nuclear processes in Wangiella dermatitidis but not yeast budding. This suggests microtubules do not control budding or multicellular development.

    Area of Science:

    • Mycology
    • Cell Biology
    • Biochemistry

    Background:

    • Wangiella dermatitidis exhibits fungal polymorphism, transitioning between yeast and multicellular forms.
    • Understanding the cell cycle regulation in fungi is crucial for controlling dimorphism.

    Purpose of the Study:

    • To investigate the role of microtubules in the cell cycle of Wangiella dermatitidis.
    • To determine if microtubule function is essential for yeast budding and multicellular development.

    Main Methods:

    • Treatment of Wangiella dermatitidis with the microtubule inhibitor nocodazole.
    • Microscopic observation of nuclear migration, nuclear division, and bud formation.
    • Analysis of cell cycle progression and developmental pathways.

    Main Results:

    • Nocodazole inhibited nuclear migration and division in both yeast and multicellular forms.
    • Yeast bud formation continued for several cycles despite nocodazole treatment.
    • Cells accumulated in premitotic stages with multiple buds, indicating a specific cell cycle block.
    • The nocodazole block point was distinct from pathways regulated by temperature-sensitive mutations affecting multicellularity.

    Conclusions:

    • Microtubules are not essential for initiating yeast budding in Wangiella dermatitidis.
    • Microtubule function does not appear to regulate the development of multicellular forms in this fungus.
    • The findings suggest complex cell cycle control pathways independent of direct microtubule involvement in budding and development.

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