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Type-specific collagen degradation by eosinophils

M S Hibbs, C L Mainardi, A H Kang

    The Biochemical Journal
    |December 1, 1982
    PubMed
    Summary
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    Eosinophils possess a metalloprotein capable of degrading types I and III collagen. This finding clarifies the role of eosinophils in connective tissue remodeling during healing and fibrosis.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Eosinophils are implicated in wound healing and fibrosis.
    • Their specific collagen-degrading capacity remains unclear.

    Purpose of the Study:

    • To investigate the collagenolytic activity of eosinophils.
    • To determine the types of collagen degraded by eosinophils.

    Main Methods:

    • Purified eosinophil preparations (>95% purity) from guinea pigs.
    • Assays for type-specific collagen degradation.
    • Use of 4-aminophenylmercuric acetate to activate latent enzymes.

    Main Results:

    • Eosinophils contain a metalloprotein that degrades type I and type III collagens.

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  • Collagenolytic activity was latent, requiring activation.
  • No degradation of type IV or type V collagens was observed.

    • Conclusions:

    • Eosinophils possess latent collagenase activity against specific collagen types.
    • This activity may contribute to connective tissue matrix alterations in physiological and pathological conditions.