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Experimental "Runde" virus infections in embryonated eggs and chickens
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology
|October 1, 1978
Summary
Runde virus infection in chicks and embryonated eggs caused neurological symptoms and reduced mouse pathogenicity. This study explored Runde virus adaptation in avian hosts.
Area of Science:
- Virology
- Pathogen Adaptation
- Avian Health
Background:
- Runde virus is a pathogen with known mouse pathogenicity.
- Understanding viral adaptation in different hosts is crucial for disease control.
Purpose of the Study:
- To investigate the effects of Runde virus inoculation in 3-day-old chicks and embryonated eggs.
- To assess the virus's pathogenicity and replication in avian models.
- To evaluate the impact of avian passage on mouse pathogenicity.
Main Methods:
- Inoculation of 3-day-old chicks and 11-12 day embryonated eggs with 100 BMLD50 of Runde virus.
- Infection routes included subcutaneous (chicks), allantoic/amniotic cavities, yolk sac, and chorioallantoic membrane (eggs).
- Monitoring for viraemia, antibody response, viral replication in specific egg compartments, and neurological signs.
Main Results:
- Runde virus induced viraemia and antibody responses in chicks.
- Viral multiplication was confined to the amniotic cavity in eggs, with detection in embryo brains (days 5-9 p.i.).
- Chicks from hatched eggs exhibited "epilepsy-like" attacks and high antibody titers; unhatched chicks were weak with virus in brains.
Conclusions:
- Avian passage (chicks or eggs) of Runde virus appears to reduce its pathogenicity in mice.
- No antigenic differences were observed between chick- and mouse-passaged virus strains.
- The findings suggest potential for viral adaptation and altered pathogenicity in avian hosts.