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Updated: Aug 16, 2026

Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate
Published on: April 24, 2018
Pyrrolopyridine derivatives from pyridoxal 5'-sulfate
Abstract:
A fluorescent derivative of lysine-258 isolated from the active site of aspartate aminotransferase modified by treatment of the apoenzyme with pyridoxal 5'-sulfate has been characterized as a substituted 2H-pyrrolo[3,4-c]pyridine. Similar pyrrolopyridines are produced in up to 20% yield by reaction of pyridoxal sulfate with simple alkylamines or with amino acids including lysine. The latter forms two products, one of which is identical with that isolated from the enzyme. The pyrrolopyridine derived from ethylamine has been characterized by proton and 13C NMR, ultraviolet-visible, and mass spectroscopy and by its chemical reactions.
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