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Phospholipase A activity in commercial nucleases. Implications for membrane vesicle isolation
Biochimica Et Biophysica Acta
|November 22, 1982
Summary
Commercial DNAase and RNAase preparations contain phospholipase A activity. Higher nuclease purification levels correlate with lower phospholipase A levels, impacting biological membrane isolation and enzymatic studies.
Area of Science:
- Biochemistry
- Enzymology
Background:
- Commercial nuclease preparations (DNAases and RNAases) are widely used in molecular biology and biochemistry.
- Potential contamination with other enzymatic activities can affect experimental outcomes.
Purpose of the Study:
- To investigate the presence and levels of phospholipase A activity in commercial DNAase and RNAase preparations.
- To determine the relationship between nuclease purification and phospholipase A contamination.
Main Methods:
- Enzymatic assays were performed on commercial DNAase I, DNAase II, and RNAase preparations.
- Phospholipase A activity was quantified in each preparation.
Main Results:
- Phospholipase A activity was detected in all tested commercial DNAase and RNAase preparations.
- A negative correlation was observed between the degree of nuclease purification and the amount of phospholipase A activity.
Conclusions:
- Commercial nucleases are often contaminated with phospholipase A.
- Assessing phospholipase A levels is crucial for accurate enzymatic studies and successful biological membrane isolation using these preparations.