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Stem cell (CFU-s) proliferation in sublethally irradiated mice
Cell and Tissue Kinetics
|November 1, 1982
Summary
Haemopoietic stem cell (CFU-s) proliferation is delayed after irradiation, entering DNA synthesis 14-16 hours later. This occurs in synchronized waves, not immediately as previously thought.
Area of Science:
- Stem cell biology
- Radiation biology
- Haematopoiesis
Background:
- Sublethal total body irradiation impacts haemopoietic stem cell (CFU-s) proliferation.
- Previous studies suggested immediate DNA synthesis post-irradiation.
Purpose of the Study:
- To investigate the proliferation rate of haemopoietic stem cells (CFU-s) after sublethal irradiation.
- To determine the precise timing of CFU-s entry into DNA synthesis (S-phase).
Main Methods:
- Utilized the [3H]-thymidine suicide technique to measure CFU-s proliferation.
- Conducted measurements from 10 minutes to 21 days post-irradiation.
Main Results:
- CFU-s demonstrated a delayed entry into S-phase, occurring 14-16 hours after irradiation.
- Proliferation exhibited an oscillatory pattern, with synchronized waves of cell division.
- The period of these synchronized waves was approximately 20-22 hours.
Conclusions:
- CFU-s do not immediately synthesize DNA after irradiation.
- Haemopoietic stem cell proliferation post-irradiation is characterized by delayed, synchronized, and oscillatory behavior.