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Related Experiment Videos

Superoxide dismutase in extracellular fluids

S L Marklund, E Holme, L Hellner

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |November 24, 1982
    PubMed
    Summary
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    Serum CuZn superoxide dismutase levels correlate with kidney function. A similar high-molecular-weight enzyme exists in various bodily fluids and mammals, distinct from the standard enzyme.

    Area of Science:

    • Biochemistry
    • Nephrology
    • Enzymology

    Background:

    • CuZn superoxide dismutase (SOD1) is a crucial antioxidant enzyme.
    • Its presence and role in human extracellular fluids, particularly serum, are not fully characterized.
    • Impaired renal function can affect serum enzyme levels.

    Purpose of the Study:

    • To investigate the levels and characteristics of superoxide dismutase activity in human serum.
    • To explore the relationship between serum CuZn superoxide dismutase and renal function.
    • To identify the nature of the superoxide dismutase activity in human extracellular fluids and compare it across species.

    Main Methods:

    • Measurement of CuZn superoxide dismutase and creatinine in serum from healthy volunteers and patients with impaired renal function.

    Related Experiment Videos

  • Enzyme activity assays using cyanide inhibition.
  • Size exclusion chromatography to determine molecular mass.
  • Radioimmunoassay and antibody inhibition assays to compare with known CuZn superoxide dismutase.
  • Analysis of extracellular fluids and plasma from various mammalian species.

    • Main Results:

    • Healthy serum had low CuZn superoxide dismutase; patients with impaired renal function showed higher levels, correlating with creatinine.
    • The primary serum SOD activity was cyanide-sensitive, associated with a high-molecular-mass factor (~130,000 Da).
    • This factor was present in human plasma, lymph, ascites, and cerebrospinal fluid, but was immunologically distinct from CuZn SOD1.
    • A similar high-molecular-mass SOD factor was found in plasma of all investigated mammals, with interspecies variations in activity.

    Conclusions:

    • Human serum contains a high-molecular-mass, cyanide-sensitive superoxide dismutase activity, likely originating from extracellular sources.
    • This activity is elevated in patients with impaired renal function and correlates with serum creatinine.
    • The identified factor represents a distinct form of superoxide dismutase found across mammalian extracellular fluids.