Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A fluorescence cytophotometer operated under computer control for multi-parameter cell analysis

M Fukuda, K Hoshino, M Naito

    Histochemistry
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Observation of Coulomb-Assisted Nuclear Bound State of Ξ^{-}-^{14}N System.

    Physical review letters·2021
    Same author

    Decreased Incidence of Acute Cellular Rejection in Low-Muscle-Mass Recipients After Living-donor Liver Transplantation.

    Transplantation proceedings·2018
    Same author

    <i>In vitro</i> single-unit recordings reveal increased peripheral nerve conduction velocity by focused pulsed ultrasound.

    Biomedical physics & engineering express·2018
    Same author

    Impact of Donor Age on Outcome of Intestinal Transplantation in Japan.

    Transplantation proceedings·2018
    Same author

    Three-Year Prospective Follow-up of Potential Pediatric Candidate for Intestinal Transplantation.

    Transplantation proceedings·2018
    Same author

    Phylogenetic and phylodynamic analyses of hepatitis C virus subtype 1a in Okinawa, Japan.

    Journal of viral hepatitis·2018

    A novel fluorescence cytophotometer enables automated multi-parameter cell analysis. This system allows rapid, consecutive determination of cellular constituents for advanced data retrieval and display.

    Area of Science:

    • Cell Biology
    • Biotechnology
    • Medical Instrumentation

    Background:

    • Traditional cytophotometry methods can be time-consuming and limited in multi-parameter analysis.
    • The need for automated, high-throughput cell analysis is crucial in various biological and medical fields.
    • Existing instruments may lack the flexibility for rapid, consecutive measurement of multiple cellular components.

    Purpose of the Study:

    • To introduce a new fluorescence cytophotometer designed for advanced multi-parameter cell analysis.
    • To demonstrate the instrument's capability for automated, multi-color fluorescence cytophotometry.
    • To showcase the system's utility in performing combined measurements of cellular constituents.

    Main Methods:

    • Development of a fluorescence cytophotometer (Olympus BH2-QRFL) with four interchangeable filter sets for multi-color analysis.

    Related Experiment Videos

  • Integration with a personal computer (HP 85F) for automated filter set operation and data acquisition.
  • Application of ninhydrin-Schiff and Feulgen staining techniques for simultaneous protein and DNA measurement in gynecological samples.

    • Main Results:

    • The developed cytophotometer allows for rapid and consecutive determination of multiple cellular constituents on a single-cell basis.
    • Automated operation via computer software enables efficient sequencing of filter sets for comprehensive analysis.
    • Data can be stored, retrieved, and displayed in various formats (tabular, histograms, scatter plots) for detailed statistical analysis.

    Conclusions:

    • The new fluorescence cytophotometer offers a powerful and automated solution for multi-parameter cell analysis.
    • The system facilitates efficient and detailed investigation of cellular components, exemplified by combined protein and DNA measurements.
    • This technology has significant potential for applications in research, diagnostics, and cell-based screening.