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Immune transfer using spleen fragments

R F Gagnon, I C MacLennan

    Journal of Clinical & Laboratory Immunology
    |October 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Spleen slices are as effective as spleen cell suspensions for adoptive immune transfer in rodents. This method provides comparable or superior immune responsiveness, offering a viable alternative for immune transfer studies.

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    Area of Science:

    • Immunology
    • Transplantation Biology
    • Rodent Models

    Background:

    • Adoptive immune transfer is crucial for studying immune responses.
    • Spleen cell suspensions are commonly used but may have limitations.
    • Evaluating alternative tissue preparations is necessary for optimizing transfer efficiency.

    Purpose of the Study:

    • To assess the efficacy of spleen slices versus spleen cell suspensions for adoptive immune transfer.
    • To compare immune responsiveness following transfer of different spleen preparations.
    • To determine the adequacy of spleen slices in rodent models.

    Main Methods:

    • Rodent models were used for parallel adoptive immune transfer experiments.
    • Hyperimmune donors provided spleen cell suspensions and spleen slices.

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  • Non-immune intact and irradiated syngeneic recipients were utilized.

    • Main Results:

    • Immune responsiveness with spleen slices was at least equal to that of cell suspensions.
    • In most cases, spleen slices yielded greater immune responsiveness.
    • Spleen slices proved adequate for adoptive immune transfer.

    Conclusions:

    • Spleen slices are a suitable and effective alternative to spleen cell suspensions for adoptive immune transfer.
    • This method offers comparable or enhanced immune reconstitution in recipients.
    • The findings support the use of spleen slices in immunological research.