Related Experiment Videos
Changes in the concentrations of certain biochemical parameters in the peripheral blood during exercise and
Insights
Bloodletting in young men alters key blood markers and acid-base balance, with exercise exacerbating these effects. These physiological changes persist for at least 24 hours post-donation.
Area of Science:
- Physiology
- Biochemistry
- Sports Medicine
Background:
- Blood donation is a common practice.
- Understanding the physiological impact of bloodletting, especially when combined with exercise, is crucial for donor safety and recovery.
Purpose of the Study:
- To investigate the effects of acute bloodletting on biochemical parameters and acid-base equilibrium in young men.
- To assess the impact of submaximal exercise on these parameters following bloodletting.
Main Methods:
- 18 healthy men (19-23 years) underwent bloodletting (400 ml).
- Exercise testing (Monark cycle ergometer) was performed before, 1 hour, and 24 hours after bloodletting.
- Venous blood samples analyzed for total protein, albumin, free fatty acids, glucose, lactate, pyruvate, and enzyme activity (LDH, ALT, AST).
- Capillary blood analyzed for acid-base equilibrium.
Main Results:
- Bloodletting led to decreased albumin, total protein, and free fatty acids, alongside a slight increase in glucose.
- Partial oxygen pressure decreased, carbon dioxide pressure increased, and hydrogen ion concentration rose post-bloodletting.
- Exercise after bloodletting induced similar biochemical and acid-base changes, which were more pronounced at 24 hours than 1 hour.
Conclusions:
- Acute bloodletting significantly impacts biochemical markers and acid-base balance in young men.
- Submaximal exercise amplifies these physiological disturbances, highlighting the importance of considering physical activity post-donation.
Abstract:
The reported investigations were carried out in 18 men aged 19 to 23 years in whom 400 ml of whole blood was removed. On the day before bloodletting, one hour and 24 hours after it the studied men carried out a 10 minute exercise on a Monark cycle ergometer at a workload raising the heart rate to 170/min. Before the exercise, immediately after it and in the 30th minute of restitution venous blood samples were taken for determinations of the concentrations of total protein, albumins, free fatty acids, glucose, lactate and pyruvate, and the activity of lactic dehydrogenase, alanine aminotransferase and aspartate aminotransferase. During that time the acid-base equilibrium was determined in capillary blood. After bloodletting the concentrations of albumins, total protein and free fatty acids were decreased parallelly to haematocrit value decrease (p less than 0.05) and glucose concentration increased slightly (p less than 0.05). Enzyme activity was decreased slightly (p greater than 0.05). The partial oxygen pressure decreased, that of carbon dioxide increased, and hydrogen ion concentration rose. These changes were more pronounced after 24 hours than 1 hour after bloodletting. After submaximal exercise and in the 30th minute of restitution as well as 1 and 24 hours after bloodletting the changes in the concentrations of the biochemical parameters, enzyme activity and acid-base equilibrium were similar as after bloodletting.