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Related Experiment Videos

[Stromal bone marrow cells and the hematopoietic microenvironment]

A Ia Fridenshteĭn

    Arkhiv Patologii
    |January 1, 1982
    PubMed
    Summary

    Stromal fibroblasts in bone marrow can form new bone marrow organs. These cells, when isolated and transplanted, create functional hematopoietic microenvironments independently of blood cells.

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    Biulleten' eksperimental'noi biologii i meditsiny·1999
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    [Osteogenic stem cells of the bone marrow].

    Ontogenez·1991

    Area of Science:

    • Biomedical Engineering
    • Hematology
    • Cell Biology

    Background:

    • Bone marrow microenvironment is crucial for hematopoiesis.
    • Stromal clonogenic fibroblasts possess osteogenic potential.
    • These stromal cells are distinct from hematopoietic cells.

    Purpose of the Study:

    • To investigate the osteogenic potential of bone marrow stromal fibroblasts.
    • To explore methods for isolating and culturing these cells.
    • To determine the feasibility of creating new bone marrow organs through cell transplantation.

    Main Methods:

    • Bone marrow stromal fibroblasts were isolated and cloned in cell cultures.
    • Cell suspensions were prepared using trypsinization and mechanical mincing.
    • Progeny cells were retransplanted into the body and in porous sponges.

    Main Results:

    • Stromal clonogenic fibroblasts were successfully recovered and cultured.
    • Trypsinization yielded higher concentrations of stromal clonogenic cells compared to mechanical mincing.
    • Transplantation of these cells led to the formation of new bone marrow organs, including ectopic sites.

    Conclusions:

    • Bone marrow stromal fibroblasts possess osteogenic potential and can regenerate bone marrow.
    • Cell culture and transplantation techniques offer a method for bone marrow organogenesis.
    • Optimized cell isolation methods enhance the yield of osteogenic stromal cells.

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