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Selective inhibition of human neutrophilic chemotaxis by echo virus, type 9. Virus-induced changes in membrane

B Bültmann, I Melzner, O Haferkamp

    Pathology, Research and Practice
    |January 1, 1982
    PubMed

    Insights

    Echo 9 virus reduces human white blood cell membrane fluidity, impairing their response to chemoattractant signals. This viral effect on granulocytes impacts cell function and signal processing.

    Area of Science:

    • Cell Biology
    • Virology
    • Immunology

    Background:

    • Human polymorphonuclear neutrophils (PMNs) play a crucial role in immune responses.
    • Chemoattractants like FMLP are vital for directing PMN migration.
    • Viral infections can modulate immune cell function.

    Purpose of the Study:

    • To investigate the impact of Echo 9 virus on human PMN membrane fluidity.
    • To determine how viral exposure affects PMN response to chemoattractants.
    • To elucidate the mechanism of virus-induced alterations in cell signaling.

    Main Methods:

    • Utilized the excimer-forming lipid technique to measure membrane fluidity.
    • Exposed human PMNs to FMLP and/or Echo 9 virus.
    • Analyzed dose-dependent effects of the virus on PMN membrane properties.

    Main Results:

    • Preincubation with Echo 9 virus decreased PMN membrane fluidity in a dose-dependent manner.
    • Viral exposure led to increased rigidity of the granulocyte cell membrane.
    • This virus-induced rigidity disrupted membrane particle aggregation and receptor formation.

    Conclusions:

    • Echo 9 virus significantly alters human PMN membrane fluidity.
    • The observed membrane rigidity interferes with normal chemotactic signal processing.
    • This mechanism may explain how viral infections can impair immune cell function.

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